Ancient Minister
Ancient Minister has only appeared in one game, which is Super Smash Bros. Brawl. He is being forced by Tabuu to command the R.O.B. squad. Later in the game, he turns out to be R.O.B.
Baby Donkey Kong
Baby Donkey Kong is obviously a character in the "Donkey Kong" series. He is Donkey Kong's baby form.
Baby Luigi
Obviously the baby form of Luigi, Baby Luigi has appeared in many spin-off games, one of which is Mario Kart Wii.
Baby Mario
Obviously the baby form of Mario, has appeared in many games. Like Baby Luigi, he's appeared in Mario Kart Wii, although, he's probably most famous from the Yoshi games.
Birdo
Birdo is very oddly, actually an antagonist in the Mario series. She's appeared in many Mario spin-off games like Mario Super Sluggers, Mario Kart Wii, and Mario Tennis.
Black Mage
A Black Mage is a job in the "Final Fantasy" series. They use black magic.
Blooper
Blooper's are main enemies in the Mario series. They live underwater, and obviously resemble a squid.
Boo
Boo's are another main enemy in the Mario series. They're basically the Mario version of a ghost.
Bowser
Pretty much everyone who knows what Nintendo is knows who Bowser is, because he's Mario's worst enemy. He's appeared in probably every Mario game there's been, including Mario spin-off games.
Bowser Jr.
Bowser Jr. is Bowser's son. He's appeared in a lot of Mario games, but not as many as his father. He's appeared in many Mario spin-off games.
Cactuar
Cactuars are common enemies in the "Final Fantasy" series.
Captain Falcon
Captain Falcon is a bounty hunter in the "F-Zero" series. He's appeared in every Super Smash Bros. game up to date.
Cid
Cid is a character in the "Final Fantasy" series.
Crash
Crash is the main character in the "Crash Bandicoot" series.
Daisy
Daisy is a princess in the "Mario" series. Not as well known as the other princess, Peach, but has appeared in many Mario spin-off games.
Diddy Kong
Diddy Kong is a main character in the "Donkey Kong" series. He's Donkey Kong's best friend.
Dixie Kong
Dixie Kong is a character in the "Donkey Kong" series. She's Diddy Kong's girlfriend.
Donkey Kong
Donkey Kong is obviously the main character in the "Donkey Kong" series. He's best friends with Diddy Kong.
Donkey Kong Jr.
Donkey Kong Jr. is a character in the "Donkey Kong" series. He's the son of Donkey Kong.
Dr. Mario
Dr. Mario is obviously the doctor version of Mario. He appeared in "Super Smash Bros. Melee" but didn't return for "Super Smash Bros. Brawl."
Dry Bones
Dry Bones' are antagonists in the "Mario" series. If you "kill" them, in about seven seconds they come back to "life."
Dry Bowser
Dry Bowser is pretty much the Dry Bones version of Bowser. He doesn't appear in many games, but he's been in "New Super Mario Bros." and "Mario Kart Wii."
Falco
Falco is one of the main characters in the "Star-Fox" series.
Fox
Fox is one of the main characters in the "Star-Fox" series.
Funky Kong
Funky Kong is a character in the "Donkey Kong" series.
Ganondorf
Ganondorf (a.k.a. Ganon) is the main antagonist in "The Legend of Zelda" series.
Ghost Gang
The Ghost Gang is the group of ghosts that try to defeat Pac-Man. The leader of the Ghost Gang is the red ghost, "Blinky."
Goomba
Goomba's are probably the most common and most well known antagonists in the "Mario" series (other than Bowser). They're basically the Mario version of a mushroom.
Hammer Bro.
Hammer Bros. are antagonists in the "Mario" series. They throw hammers at you to hurt you.
Ice Climbers
The Ice Climbers are the main characters in the "Ice Climber" series.
Ike
Ike is the main character in the "Fire Emblem" series.
Jigglypuff
Jigglypuff is a pokémon who is very famous for her move, "Sing." Sing makes the opponent fall asleep.
King Boo
King Boo is an antagonist in the "Mario" series. He is the leader of the Boo's.
King Dedede
King Dedede is the main antagonist of the "Kirby" series.
King K. Rool
King K. Rool is the main antagonist in the "Donkey Kong" series. He is the leader of the Kremlings.
Kirby
Kirby is the main character in the "Kirby" series. He's one of Nintendo's most succesful characters.
Koopa Troopa
Koopa Troopa's are one of the most common enemies in the "Mario" series. If you bounce on them, they go into their shell, and you can use the shell to throw at other enemies.
Kritter
Kritter's are antagonists in the "Donkey Kong" series. They're the most common of of Kremling Krew.
Lakitu
Lakitus are enemies in the "Mario" series. They ride clouds around. To attack, the throw down another enemy of Mario, (usually Spinies).
Link
Link is the main character in "The Legend of Zelda" series. He's one of Nintendo's most succesful characters.
Llyud
Llyud is a character in the "Final Fantasy" series.
Lucario
Lucario is a very recent pokémon. Many people think he replaced Mewtwo for "Super Smash Bros. Brawl."
Lucas
Lucas is a main character in the "Mother" series.
Luigi
Luigi is a main character in the "Mario" series. He's Mario's brother, and is one of Nintendo's most succesful characters.
Luma
Luma is a character in the "Mario" series. He has appeared only in the "Super Mario Galaxy" games. He goes in Mario and Luigi's hats to allow them to do special moves.
Magikoopa
Magikoopas are enemies in the "Mario" series.
Mario
Mario is obviously the main character in the "Mario" series. He's Nintendo's most succesful character. He's been in many many many games.
Marth
Marth is a character from the "Fire Emblem" series.
MC Ballyhoo
MC Ballyhoo is a character in the "Mario series, more specifically the "Mario Party" series. In "Mario Party 8" he is the creator of the Star Carnival. His hat is living and is named Big Top.
Mega Man
Mega Man is the protagonist of the "Mega Man" series.
Meta Knight
Meta Knight is a character in the "Kirby" series.
Mewtwo
Mewtwo is a very popular pokémon. It's a very rare one, and many people like it. Some people think that Mewtwo was replaced by Lucario for "Super Smash Bros. Brawl."
Mii
Mii's are digital avatars that you can create on the Wii. In many Wii games you can play as a Mii you've created. Some are "Mario Kart Wii," "Mario Super Sluggers," and "Mario & Sonic at the Olympic Games."
Monty Mole
Monty Mole's are antagonists in the "Mario" series. They throw rocks at Mario, and are pretty much afraid of him. They're pretty much the "Mario" version of a mole.
Mr. Game & Watch
Mr. Game & Watch is one of Nintendo's first characters. His first game is called "Ball."
Ms. Pac-Man
Ms. Pac-Man is Pac-Man's girlfriend. Surprisingly, she's actually appeared in two Mario Kart games.
Ness
Ness is a main character in the "Mother" series.
Noki
Noki's are characters in the "Mario" series. They first appeared in "Super Mario Sunshine." They're probably the "Mario" version of a shellfish.
Olimar
Olimar is the main character in the "Pikmin" series. As you can see in the picture, there are other creatures around him, these are called Pikmin.
Pac-Man
Pac-Man is one of the most succesful video-game characters of all time. He is a Pac-Person and is the boyfriend of Ms. Pac-Man.
Peach
Peach is a main character in the "Mario" series. She is the main princess, more popular and well known than Daisy. She is Mario's girlfriend.
Petey Piranha
Petey Piranha is an antagonist in the "Mario" series. He is a boss in many games, including "Super Mario Galaxy," and "Super Smash Bros. Brawl."
Pianta
Piantas are protagonists in the "Mario" series. Their first appearance was in "Super Mario Sunshine."
Pichu
Pichu is a pokémon. Pichu evolves into probably the most famous pokémon, Pikachu.
Pikachu
Pikachu is probably the most famous pokémon. The main character in the "Pokémon" series is Ash Ketchum. Pikachu was Ash's first pokémon, and probably his favorite.
Pit
Pit is the main character in the "Kid Icarus" series.
Pokémon Trainer
The Pokémon Trainer has three pokémon, Squirtle, Ivysaur, and Charizard. You can't actually fight as the Pokémon Trainer himself though.
Primid
Primids are the most common enemies in the "Subspace Emissary." "Super Smash Bros. Brawl" is the only game the Primid has appeared in.
Psyduck
Psyduck is a pokémon who is thought as to be weak. Although, they have shown in the anime that Psyduck can use all of his "headaches" and turn them into a super powerful psychic blast.
R.O.B.
R.O.B. was originally a robot that could substitute for a second player in the games "Stack Up" and "Gyromite." He has now appeared in "Mario Kart DS" and "Super Smash Bros. Brawl."
Rosalina
Rosalina is a princess in the "Mario" series who's first game was "Super Mario Galaxy." She is the mother of all Lumas. (Star shaped creatures in "Super Mario Galaxy"). She's also appeared in "Mario Kart Wii."
Roy
Roy is a character in the "Fire Emblem" series.
Samus
Samus is the main character in the "Metroid" series.
Shadow
Shadow is a character from the "Sonic the Hedgehog" series. He started out as a protagonist, but later in the series, they changed him into an antagonist.
Sheik
Sheik is a character from "The Legend of Zelda" series. She is another form of Zelda.
Shy Guy
Shy Guy's are enemies in the "Mario" series.
Snake
Snake is the main character in the "Metal Gear" series.
Sonic
Sonic is the main character in the "Sonic the Hedgehog" series. "Sega" created him. Sonic is a very well known video-game character.
Tiny Kong
Tiny Kong is a character in the "Donkey Kong" series. She's Dixie Kong's younger sister.
Toad
Toad is a character in the "Mario" series. He helps protect Peach from harms way. (Mainly Bowser).
Toadette
Toadette is a female Toad in the "Mario" series.
Toadsworth
Toadsworth is a very elderly Toad in the "Mario" series. He is Peach's servant and is very protective of her. He acts as a father to her.
Toon Link
Toon Link is basically, a more toonier version of Link. His only appearance is in "Super Smash Bros. Brawl."
Waluigi
Waluigi is an antagonist in the "Mario" series.
Wario
Wario is an antagonist in the "Mario" series. After he came into the "Mario" series, later on, there became a "Wario" series. In the "Wario" series, he is a protagonist.
White Mage
A White Mage is a job class in the "Final Fantasy" series. They use White Magic.
Wiggler
Wigglers are enemies in the "Mario" series. They're basically the Mario version of a caterpillar.
Wolf
Wolf is a character in the "Star-Fox" series. He is the enemy of Fox.
Yoshi
Yoshi is a character in the "Mario" series and the "Yoshi" series.
Young Link
Young Link is a character in "The Legend of Zelda" series. He is Link only as a young boy.
Zelda
Zelda is a character in "The Legend of Zelda" series.
Zero Suit Samus
Zero Suit Samus is a character in the "Metroid" series. She is basically Samus only without the power suit.